4JYC
MeaB, A Bacterial Homolog of MMAA, in its Apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03262 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.463, 58.377, 155.280 |
| Unit cell angles | 90.00, 110.07, 90.00 |
Refinement procedure
| Resolution | 48.620 - 2.200 |
| R-factor | 0.19941 |
| Rwork | 0.197 |
| R-free | 0.25217 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qm7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.398 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.124 | 0.562 |
| Number of reflections | 77430 | |
| <I/σ(I)> | 11.78 | 3.24 |
| Completeness [%] | 96.9 | 93.4 |
| Redundancy | 3.6 | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 28% PEG400, 50 mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
