4JYB
MeaB, A Bacterial Homolog of MMAA, Bound to GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03262 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.561, 58.231, 76.441 |
| Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
| Resolution | 44.540 - 2.100 |
| R-factor | 0.20089 |
| Rwork | 0.199 |
| R-free | 0.24156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qm7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.388 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.375 |
| Number of reflections | 46098 | |
| <I/σ(I)> | 14.34 | 4.29 |
| Completeness [%] | 99.1 | 98.1 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 28% PEG550MME, 50 mM MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
