4JYA
Crystal structures of pseudouridinilated stop codons with ASLs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2012-08-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 402.320, 402.320, 174.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.930 - 3.098 |
| R-factor | 0.1853 |
| Rwork | 0.183 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.748 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.930 | 3.178 |
| High resolution limit [Å] | 3.098 | 3.098 |
| Number of reflections | 254809 | |
| Completeness [%] | 99.0 | 98.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 277.15 | 12% MPD, 0.1M MES-KOH, 0.075M magnesium chloride, 0.1M KCL, pH 6.5, EVAPORATION, temperature 277.15K |
