4JY6
Crystal structure of human Fab PGT123, a broadly reactive and potent HIV-1 neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.890, 80.320, 90.430 |
Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
Resolution | 29.141 - 2.500 |
R-factor | 0.2099 |
Rwork | 0.207 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3muh |
RMSD bond length | 0.011 |
RMSD bond angle | 1.226 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 42069 | |
<I/σ(I)> | 9.2 | 2.3 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.16 M zinc acetate, 0.08 M sodium cacodylate, pH 6.5, 20% v/v glycerol, 20% w/v PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |