4JXY

CRYSTAL STRUCTURE OF POLYPRENYL SYNTHASE PATL_3739 (TARGET EFI-509195) FROM Pseudoalteromonas atlantica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 41 3 2
Unit cell lengths	149.173, 149.173, 149.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.770 - 1.900
R-factor	0.17463
Rwork	0.174
R-free	0.19743
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gp1
RMSD bond length	0.009
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.080
Number of reflections	45233
<I/σ(I)>	6.5	2.8
Completeness [%]	100.0	100
Redundancy	33.7	34.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.2M HEPES:NAOH, PH 7.5, 0.8 M NaH2PO4, 0.8 M KH2PO4, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K