4JXE
Crystal structure of Schizosaccharomyces pombe sst2 catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-07 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.139, 69.436, 62.018 |
| Unit cell angles | 90.00, 104.84, 90.00 |
Refinement procedure
| Resolution | 29.640 - 1.451 |
| R-factor | 0.1642 |
| Rwork | 0.163 |
| R-free | 0.19240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2znr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP ((ccp4)) |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.640 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | |
| Number of reflections | 80642 | |
| <I/σ(I)> | 19.28 | |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M Ammonium phosphate dibasic, 20 % w/v polyethylene glycol 3,350 , 30% w/v 1,6- hexanediol , VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8 |
