4JX2
Crystal structure of a putative secreted protein (lpg1979) from Legionella pneumophila subsp. pneumophila str. Philadelphia 1 at 2.65 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97932,0.91837,0.97858 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 121.943, 145.086, 63.947 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.970 - 2.650 |
R-factor | 0.1642 |
Rwork | 0.162 |
R-free | 0.20140 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | XSCALE (July 4, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.970 | 2.740 | |
High resolution limit [Å] | 2.650 | 5.670 | 2.650 |
Rmerge | 0.087 | 0.039 | 0.570 |
Number of reflections | 33147 | 3554 | 3064 |
<I/σ(I)> | 11.63 | 25.3 | 2.3 |
Completeness [%] | 98.1 | 96.3 | 97.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M lithium sulfate, 40.0% polyethylene glycol 400, 0.2M lithium sulfate, 40.0% polyethylene glycol 400, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |