4JWO
The crystal structure of a possible phosphate binding protein from Planctomyces limnophilus DSM 3776
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97899 |
Spacegroup name | P 61 |
Unit cell lengths | 87.158, 87.158, 75.536 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.529 - 1.601 |
R-factor | 0.1727 |
Rwork | 0.172 |
R-free | 0.19460 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.070 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.600 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.070 | 0.633 |
Number of reflections | 42889 | |
<I/σ(I)> | 50.8 | 3.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.3 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 2.0M Ammonium Sulfate, 0.1M Sodium Acetate:HCl, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |