4JWK
Crystal structure of the complex of peptidyl-tRNA hydrolase from Acinetobacter baumannii with cytidine at 1.87 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-11-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.257, 64.391, 78.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.570 - 1.870 |
| R-factor | 0.18072 |
| Rwork | 0.179 |
| R-free | 0.22156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iko |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.570 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 14269 | |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M HEPES, 25% PEG400, 12% PEG1500, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
