4JWJ

Crystal structure of scTrm10(84)-SAH complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-06
Detector	RAYONIX MX-225
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	131.583, 58.861, 99.767
Unit cell angles	90.00, 131.13, 90.00

Refinement procedure

Resolution	37.570 - 1.760
R-factor	0.2195
Rwork	0.218
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jwf
RMSD bond length	0.013
RMSD bond angle	1.396
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.820
High resolution limit [Å]	1.760	3.790	1.760
Rmerge	0.066	0.038	0.507
Number of reflections	56871
<I/σ(I)>	14.5
Completeness [%]	99.7	97.8	100
Redundancy	3.7	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	287	1.5M Lithium sulfate monohydrate, 0.1M BIS-TRIS propane, pH 7.0, vapor diffusion, hanging drop, temperature 287K