4JWG
Crystal structure of spTrm10(74)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.677, 69.568, 121.076 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.480 - 2.500 |
| R-factor | 0.2145 |
| Rwork | 0.211 |
| R-free | 0.28390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jwf |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.727 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.090 | 0.045 | 0.492 |
| Number of reflections | 7871 | ||
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 99.8 | 99.2 | 100 |
| Redundancy | 7 | 6.5 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 287 | 15% PEG 6000, 0.1M Na acetate, pH 5.0, vapor diffusion, hanging drop, temperature 287K |
