4JWF

Crystal structure of spTrm10(74)-SAH complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-27
Detector	RAYONIX MX-225
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	51.570, 73.160, 119.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.350 - 2.400
R-factor	0.21883
Rwork	0.216
R-free	0.26169
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.215
Data reduction software	MOSFLM (3.3.20)
Data scaling software	SCALA (3.3.20)
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.890	51.890	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.062	0.449
Number of reflections	18531
<I/σ(I)>	16.2	10.4	1.4
Completeness [%]	99.9	97.6	100
Redundancy	13.6	10.1	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	287	15% PEG 6000, 0.1M Na acetate, pH 5.0, vapor diffusion, hanging drop, temperature 287K