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4JWE

Crystal structure of the substrate binding domain of E.coli DnaK in complex with sheep Bac7(1-21)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBESSY BEAMLINE 14.1
Synchrotron siteBESSY
Beamline14.1
Temperature [K]100
Detector technologyCCD
Collection date2011-04-29
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.918
Spacegroup nameP 21 21 2
Unit cell lengths77.659, 162.531, 44.783
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution24.670 - 1.950
R-factor0.1946
Rwork0.193
R-free0.22640
Structure solution methodFOURIER SYNTHESIS
Starting model (for MR)3dpo
RMSD bond length0.010
RMSD bond angle1.100
Data reduction softwareXDS
Data scaling softwareSCALA
Refinement softwareBUSTER (2.8.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]24.6702.060
High resolution limit [Å]1.9501.950
Rmerge0.0490.273
Number of reflections39570
Completeness [%]93.598.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP52922.4 M ammonium sulfate, 0.1 M sodium acetate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K

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