4JWE
Crystal structure of the substrate binding domain of E.coli DnaK in complex with sheep Bac7(1-21)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.659, 162.531, 44.783 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.670 - 1.950 |
R-factor | 0.1946 |
Rwork | 0.193 |
R-free | 0.22640 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3dpo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.670 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.049 | 0.273 |
Number of reflections | 39570 | |
Completeness [%] | 93.5 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 2.4 M ammonium sulfate, 0.1 M sodium acetate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |