4JW0

Structure of Gloeobacter violaceus CcmL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	74.377, 81.619, 89.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.297 - 1.700
R-factor	0.1624
Rwork	0.161
R-free	0.19140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.944
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	1.800
High resolution limit [Å]	1.700	1.700
Rmerge		0.014
Number of reflections	58606
<I/σ(I)>		0.9
Completeness [%]	97.4	82.4
Redundancy		3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	0.2 M Lithium sulfate, 0.1 M Bis-Tris, 10% (w/v) PEG 3350, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K