4JVY

Structure of the STAR (signal transduction and activation of RNA) domain of GLD-1 bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Detector technology	CCD
Collection date	2012-04-23
Detector	ADSC QUANTUM 315r
Spacegroup name	P 31 2 1
Unit cell lengths	142.274, 142.274, 76.652
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.900 - 2.853
R-factor	0.1797
Rwork	0.175
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.108
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.950
High resolution limit [Å]	2.850	6.090	2.850
Rmerge	0.076	0.032	0.654
Number of reflections	20941
<I/σ(I)>	12
Completeness [%]	99.5	98	99.9
Redundancy	5.6	5.4	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5		SODIUM ACETATE, PH 6.5, VAPOR DIFFUSION