4JV5

Crystal structures of pseudouridinilated stop codons with ASLs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	77
Detector technology	CCD
Collection date	2012-08-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	401.000, 401.000, 176.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.880 - 3.162
R-factor	0.20002
Rwork	0.197
R-free	0.24905
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.698
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0016)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	3.300
High resolution limit [Å]	3.100	3.100
Number of reflections	241499
<I/σ(I)>		1.8
Completeness [%]	99.2	98.7
Redundancy		2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	277.15	12%MPD 0.1M MES-KOH pH6.5 50mM KCL 10mM NH4-CL 15mM Mg2CL, EVAPORATION, temperature 277.15K