4JUT
Crystal structure of a mutant fragment of Human HSPB6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.440, 86.010, 87.070 |
| Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
| Resolution | 43.005 - 2.196 |
| R-factor | 0.1991 |
| Rwork | 0.196 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.164 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.010 |
| High resolution limit [Å] | 2.196 |
| Number of reflections | 49462 |
| Completeness [%] | 99.4 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor diffusion | 7.5 | 277 | 0.1M HEPES (pH 7.5), 0.2M litium nitrate, 20% PEG 3350, hanging drop vapor diffusion, temperature 277K |
