4JUL
Crystal structure of H5N1 influenza virus hemagglutinin, clade 2.3.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.584, 71.557, 247.054 |
| Unit cell angles | 83.94, 85.46, 60.68 |
Refinement procedure
| Resolution | 48.873 - 2.793 |
| R-factor | 0.1989 |
| Rwork | 0.196 |
| R-free | 0.25410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jum |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.439 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.793 | 6.030 | 2.793 |
| Rmerge | 0.092 | 0.042 | 0.379 |
| Number of reflections | 85955 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 81.8 | 99.4 | 68 |
| Redundancy | 2.6 | 3.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 19% PEG 6000, 0.2M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
