4JUK
Crystal structure of H5N1 influenza virus hemagglutinin, clade 2.3.2.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 116.365, 116.365, 134.596 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.189 - 2.750 |
R-factor | 0.2306 |
Rwork | 0.229 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jum |
RMSD bond length | 0.014 |
RMSD bond angle | 1.351 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.750 | 5.920 | 2.750 |
Rmerge | 0.150 | 0.089 | 0.531 |
Number of reflections | 27769 | ||
<I/σ(I)> | 10.1 | ||
Completeness [%] | 99.5 | 99.3 | 96.6 |
Redundancy | 6.4 | 6.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 22% PEG 4000, 0.2M LiSO4, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 22% PEG 4000, 0.2M LiSO4, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |