4JUE

Crystal structure of Plasmodium falciparum ubiquitin conjugating enzyme UBC9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	28.870, 94.390, 124.890
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	24.978 - 1.851
R-factor	0.1491
Rwork	0.146
R-free	0.18830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Balbes automatic selection
RMSD bond length	0.007
RMSD bond angle	1.599
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.850
Number of reflections	53373
Completeness [%]	93.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M NaF, 20% PEG3350 Cryo: 30% PEG3350, 10% glycerol, 0.2M NaF, 100mM glycine, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K