4JTO

Cysteine bound Cysteine Dioxygenase at pH 8.0 in the presence of Cys and dithionite

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	77
Detector technology	IMAGE PLATE
Collection date	2013-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	57.600, 57.600, 122.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.034 - 2.000
R-factor	0.1814
Rwork	0.176
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.370
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.000
High resolution limit [Å]	2.000
Number of reflections	13313
Completeness [%]	90.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M tri-sodium citrate pH=5.6, 24% PEG 4000, 0.15M ammonium acetate. 1:1 drop ratio with microseeding, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K