4JTN
Cysteine Dioxygenase at pH 8.0 in the presence of dithionite
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-02-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.600, 57.600, 122.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.800 - 1.593 |
R-factor | 0.1735 |
Rwork | 0.171 |
R-free | 0.19920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.376 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.000 |
High resolution limit [Å] | 1.593 |
Number of reflections | 25267 |
Completeness [%] | 89.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M tri-sodium citrate pH=5.6, 24% PEG 4000, 0.15M ammonium acetate. 1:1 drop ratio with microseeding., pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |