4JT6
structure of mTORDeltaN-mLST8-PI-103 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2011-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 139.400, 163.200, 207.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.670 - 3.600 |
R-factor | 0.2397 |
Rwork | 0.239 |
R-free | 0.27490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.217 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 3.730 |
High resolution limit [Å] | 3.600 | 7.740 | 3.600 |
Rmerge | 0.060 | 0.650 | |
Number of reflections | 49787 | ||
Completeness [%] | 89.0 | 98.5 | 76.9 |
Redundancy | 4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 8000, NaCl, pH 8.5, hanging drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP |