4JT5
mTORdeltaN-mLST8-pp242 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 139.400, 163.200, 207.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.670 - 3.450 |
| R-factor | 0.23428 |
| Rwork | 0.233 |
| R-free | 0.27104 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.259 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.570 |
| High resolution limit [Å] | 3.450 | 7.420 | 3.450 |
| Rmerge | 0.043 | 0.629 | |
| Number of reflections | 61700 | ||
| Completeness [%] | 97.0 | 96.7 | 91.2 |
| Redundancy | 3.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 8000, NaCl , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
