4JS2
Crystal structure of human Beta-galactoside alpha-2,6-sialyltransferase 1 in complex with CMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 61 |
Unit cell lengths | 64.027, 64.027, 160.571 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.510 - 2.300 |
R-factor | 0.1774 |
Rwork | 0.175 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4js1 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1327)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 16396 | |
<I/σ(I)> | 11.8 | 1 |
Completeness [%] | 99.3 | 98.7 |
Redundancy | 10.3 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 0.1M HEPES/NaOH, pH 7.0, 20% PEG 550 MME, 0.01M CaCl2, 0.01M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 294K |