4JRU
Structure of haze forming proteins in white wines: Vitis vinifera thaumatin-like proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.956639 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.801, 70.168, 74.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.620 - 1.200 |
| R-factor | 0.16735 |
| Rwork | 0.166 |
| R-free | 0.18444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4H8T |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.345 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.907 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.070 | |
| Number of reflections | 51588 | |
| <I/σ(I)> | 7 | |
| Completeness [%] | 88.4 | 49.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Na acetate pH 4.6, 6% PEG 4000, 10 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
