4JRN
ROP18 kinase domain in complex with AMP-PNP and sucrose
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918, 1.53500, 1.53570, 1.53450 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.650, 48.650, 293.746 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.545 - 2.710 |
| R-factor | 0.2348 |
| Rwork | 0.232 |
| R-free | 0.28950 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.602 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.068 | 0.376 |
| Number of reflections | 12398 | |
| <I/σ(I)> | 18.4 | 4.2 |
| Completeness [%] | 91.5 | |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.56 M ammonium sulfate, 15% PEG 3350, 0.1 M Bis-Tris, 20 mM IPTG, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
