4JRN

ROP18 kinase domain in complex with AMP-PNP and sucrose

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918, 1.53500, 1.53570, 1.53450
Spacegroup name	P 31 2 1
Unit cell lengths	48.650, 48.650, 293.746
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.545 - 2.710
R-factor	0.2348
Rwork	0.232
R-free	0.28950
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.602
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.068	0.376
Number of reflections	12398
<I/σ(I)>	18.4	4.2
Completeness [%]	91.5
Redundancy	4.9	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.56 M ammonium sulfate, 15% PEG 3350, 0.1 M Bis-Tris, 20 mM IPTG, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K