4JRK

Crystal Structure of Escherichia coli Hfq Surface Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 2 21 21
Unit cell lengths	33.410, 66.309, 85.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.430 - 1.894
R-factor	0.1788
Rwork	0.177
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.213
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	CCP4
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	24.430
High resolution limit [Å]	1.894
Number of reflections	15335

Crystallization Conditions

crystal ID	method	pH	temperature	details
1