4JRF
Crystal structure of a putative cell adhesion protein (BACOVA_01548) from Bacteroides ovatus ATCC 8483 at 1.98 A resolution (PSI Community Target, Nakayama)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979029,0.979338,0.953725 |
Spacegroup name | I 4 |
Unit cell lengths | 134.022, 134.022, 57.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.384 - 1.980 |
R-factor | 0.1567 |
Rwork | 0.155 |
R-free | 0.17810 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | XSCALE (March 15, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.384 | 2.050 | |
High resolution limit [Å] | 1.980 | 4.250 | 1.980 |
Rmerge | 0.099 | 0.010 | 0.010 |
Number of reflections | 35859 | 3724 | 3507 |
<I/σ(I)> | 13.29 | 36.4 | 1.9 |
Completeness [%] | 100.0 | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M lithium sulfate, 30.0% polyethylene glycol 4000, 0.1M tris hydrochloride pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |