4JR8
Crystal structure of cruxrhodopsin-3 from Haloarcula vallismortis at 2.3 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 106.210, 106.210, 60.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.254 |
| Rwork | 0.245 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 | 0.448 |
| Number of reflections | 15610 | |
| <I/σ(I)> | 21.2 | 4.4 |
| Completeness [%] | 90.0 | 92.4 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 288 | 2.1M ammonium sulfate, 0.1M sodium citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
