4JQE
Crystal structure of scCK2 alpha in complex with AMPPN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.307, 69.707, 94.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.720 - 1.770 |
| R-factor | 0.20637 |
| Rwork | 0.204 |
| R-free | 0.24649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.666 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.770 |
| Number of reflections | 36707 |
| Completeness [%] | 91.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 2mM AMPPNP, 200mM magnesium chloride, 25% w/v polyethylene glycol 4000, 100mM sodium cacodylate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
