4JQD

Crystal structure of the Restriction-Modification Controller Protein C.Csp231I OL operator complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	120
Detector technology	CCD
Collection date	2010-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.93930
Spacegroup name	C 1 2 1
Unit cell lengths	82.120, 128.070, 78.540
Unit cell angles	90.00, 99.99, 90.00

Refinement procedure

Resolution	35.060 - 2.750
R-factor	0.20672
Rwork	0.205
R-free	0.23532
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lfp
RMSD bond length	0.012
RMSD bond angle	0.863
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	2.940
High resolution limit [Å]	2.750	2.750
Rmerge	0.075	0.304
Number of reflections	20336
<I/σ(I)>	9	2.9
Completeness [%]	97.6	89
Redundancy	3.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Protein was dialysed against the buffer containing 0.1 M NaCl, 50 mM TRIS-HCl pH 8.2, 1 mM DTT, and 1 mM EDTA. Crystallisation conditions: 0.2 M sodium nitrate, 0.1 M Bis-Tris-Propane pH 7.5, 24 % (w/v) PEG3350, protein dimer/DNA molar ratio 1:1, protein concentration 1.46 mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 289K