4JPX
Crystal structure of phenylalanine hydroxylase S203P mutant from Chromobacterium violaceum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-02-23 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 |
Unit cell lengths | 36.989, 38.569, 47.871 |
Unit cell angles | 76.66, 73.21, 85.53 |
Refinement procedure
Resolution | 22.740 - 1.550 |
R-factor | 0.15174 |
Rwork | 0.149 |
R-free | 0.20167 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ltu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.245 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.040 | |
Number of reflections | 31409 | |
<I/σ(I)> | 15.5 | |
Completeness [%] | 92.7 | 81.1 |
Redundancy | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Na-HEPES, 0.001 M Magnesium chloride hexahydrate, 0.005 M Nickel (II) chloride hexahydrate, 15% w/v PEG 3,350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |