4JPR

Structure of the ASLV fusion subunit core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-16
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	42.544, 42.544, 119.982
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.220 - 2.001
R-factor	0.2103
Rwork	0.207
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mof
RMSD bond length	0.014
RMSD bond angle	1.363
Data reduction software	d*TREK (9.9.9.1D)
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.220	35.220	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.026	0.019	0.308
Total number of observations		3401	1130
Number of reflections	5371
<I/σ(I)>	28.6	83.1	2.7
Completeness [%]	97.9	100	81.8
Redundancy	5.28	5.83	2.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	20% (w/v) MPD, 5% (w/v) PEG 8000, 0.1 M sodium cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K