4JPP
Bacteriophage phiX174 H protein residues 143-282
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00718 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 68.699, 68.699, 372.596 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.2477 |
Rwork | 0.245 |
R-free | 0.29010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.322 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
Rmerge | 0.049 | 0.327 | |
Number of reflections | 35261 | ||
<I/σ(I)> | 4.3 | ||
Completeness [%] | 96.5 | 91.7 | 99.5 |
Redundancy | 6.7 | 6.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10-15% w/v PEG8000, 0.1 M Tris-HCl, pH 7.5, 20% w/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |