4JPJ
Crystal structure of the germline-targeting HIV-1 gp120 engineered outer domain, eOD-GT6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.960, 217.730, 44.990 |
| Unit cell angles | 90.00, 119.97, 90.00 |
Refinement procedure
| Resolution | 38.970 - 2.500 |
| R-factor | 0.21242 |
| Rwork | 0.210 |
| R-free | 0.26159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TYG CHAIN A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 25751 | |
| <I/σ(I)> | 8.1 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 20% w/v PEG3350, 0.2 M magnesium acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
