4JPF
Structure of wild type Pseudomonas aeruginosa FabF (KASII) in Complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-02-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.600, 71.600, 140.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.410 - 1.670 |
| R-factor | 0.17103 |
| Rwork | 0.169 |
| R-free | 0.20212 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b7v MonomerA |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.957 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.410 | 1.770 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 43280 | |
| <I/σ(I)> | 2.15 | |
| Completeness [%] | 99.9 | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M Magnesium Chloride, 0.1 M TRIS, 10% (w/v) PEG8000, 5 mM F24, 5% DMSO, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
