4JP7
High resolution structure of a papaya barwin-like protein (crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-07 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.80 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.908, 56.964, 74.202 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 1.050 |
| R-factor | 0.14393 |
| Rwork | 0.143 |
| R-free | 0.17011 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.041 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.110 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.040 | 0.582 |
| Number of reflections | 117408 | |
| <I/σ(I)> | 12.25 | 1.94 |
| Completeness [%] | 94.8 | 92.8 |
| Redundancy | 1.98 | 1.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 2M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
