4JP6
High resolution structure of a papaya barwin-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-28 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.80 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.688, 54.971, 73.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.750 - 1.000 |
| R-factor | 0.1535 |
| Rwork | 0.152 |
| R-free | 0.18066 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.921 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.950 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.047 | |
| Number of reflections | 79816 | |
| <I/σ(I)> | 20.52 | 3.17 |
| Completeness [%] | 97.3 | 83.5 |
| Redundancy | 6.05 | 3.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 2M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
