4JP2
Crystal Structure of TT0495 protein from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-02-18 |
Detector | RIGAKU |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 62.944, 62.944, 111.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.440 - 1.150 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.150 | 1.150 |
Number of reflections | 79896 | |
Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.1 | 298 | 15% PEG 4000, 0.1M potassium phosphate, 0.1M magnesium chloride, pH 7.1, EVAPORATION, temperature 298.0K |