4JP1
Mg2+ bound structure of Vibrio Cholerae CheY3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-18 |
| Detector | BRUKER SMART 2000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 68.020, 68.020, 74.423 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.390 - 2.460 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3to5 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.600 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.450 |
| Number of reflections | 4716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 6000, 0.1M tris-Cl, pH=8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
