4JOX
Crystal structure of cry34Ab1 protein at 2.15 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 100.561, 100.561, 56.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.150 |
| R-factor | 0.23829 |
| Rwork | 0.234 |
| R-free | 0.27912 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.671 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.091 | 0.291 |
| Number of reflections | 14955 | |
| <I/σ(I)> | 17.7 | 3.5 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 19.5 | 19.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.4 | 289 | 25% (V/V) PEG400, 50mM sodium acetate, pH 4.4, LIQUID DIFFUSION, temperature 289K |
