4JOQ
Putative ribose ABC transporter, periplasmic solute-binding protein from Rhodobacter sphaeroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 86.096, 86.096, 199.986 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.080 - 1.900 |
R-factor | 0.1257 |
Rwork | 0.125 |
R-free | 0.14250 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.660 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.100 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.097 | 0.056 | 0.963 |
Number of reflections | 68031 | ||
<I/σ(I)> | 10 | 2.23 | |
Completeness [%] | 99.9 | 98.9 | 100 |
Redundancy | 10.4 | 9.2 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.2 M calcium acetate, 0.1 M Tris buffer, 20% PEG-3000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |