4JON

Crystal structure of a centrosomal protein 170kDa, transcript variant beta (CEP170) from Homo sapiens at 2.15 A resolution (PSI Community Target, Sundstrom)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-23
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	95.040, 183.332, 97.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.634 - 2.150
R-factor	0.1837
Rwork	0.183
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	PHASER (2.3.0)
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.634		2.150
High resolution limit [Å]	2.150	9.390	2.100
Rmerge	0.101	0.011	0.011
Number of reflections	46447	616	3584
<I/σ(I)>	14.07	56.7	1.3
Completeness [%]	99.6	97.5	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	59% 2-methyl-2,4-pentanediol, 0.1M Bicine pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K