4JOM
Structure of E. coli Pol III 3mPHP mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.429, 98.944, 139.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.945 - 2.900 |
R-factor | 0.1966 |
Rwork | 0.194 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hnh |
RMSD bond length | 0.003 |
RMSD bond angle | 0.733 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.945 | 3.070 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 26274 | |
Completeness [%] | 99.6 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 10-15 mg/mL protein, 15-20% PEG3350, 0.2-0.4 M sodium phosphate monobasic, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, temperature 293K |