4JOD
Crystal structure of human lysophosphatidic acid phosphatase type 6 complexed with Tris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.045, 90.708, 95.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.390 - 2.210 |
| R-factor | 0.1704 |
| Rwork | 0.169 |
| R-free | 0.20100 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.996 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.095 | 0.066 | 0.457 |
| Rmeas | 0.095 | ||
| Number of reflections | 23688 | ||
| <I/σ(I)> | 16.907 | ||
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 7 | 6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.02M citric acid, 0.08M bis-tris propane, 16% PEG3350, pH 8.0, vapor diffusion, hanging drop, temperature 289K |
