4JO7
Crystal structure of the human Nup49CCS2+3* Nup57CCS3* complex with 2:2 stoichiometry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9805 |
Spacegroup name | P 1 |
Unit cell lengths | 47.689, 47.658, 71.324 |
Unit cell angles | 88.87, 83.37, 81.42 |
Refinement procedure
Resolution | 47.125 - 1.752 |
R-factor | 0.1873 |
Rwork | 0.186 |
R-free | 0.21440 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.804 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.8_1069) |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.750 |
Number of reflections | 117121 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M MES, pH 6.5, 6.7% PEG20000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |