4JO6
Streptavidin complex with SBP-Tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 |
| Unit cell lengths | 57.523, 57.523, 177.552 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.540 - 1.750 |
| R-factor | 0.20798 |
| Rwork | 0.206 |
| R-free | 0.24201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1swe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.188 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.066 | 0.424 |
| Number of reflections | 52299 | |
| <I/σ(I)> | 29.2 | 2.5 |
| Completeness [%] | 95.3 | 72.6 |
| Redundancy | 7.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 56% Tacsimate, 12%(w/v) glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
