4JO4
Crystal structure of rabbit mAb R20 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.649, 119.690, 135.337 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.268 - 2.270 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.088 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.270 | 2.310 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.175 | 1.105 |
| Number of reflections | 53548 | |
| <I/σ(I)> | 22.55 | 3.8 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 8.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 1.4 M ammonium sulfate, 1.7% PEG400, 85 mM HEPES, pH 7.5, 15% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
