4JNX

Crystal structure of RNA silencing suppressor p19 complexed with double-helical RNA 20mer pG(CUG)6C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98035
Spacegroup name	H 3
Unit cell lengths	89.917, 89.917, 148.353
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.300 - 1.950
R-factor	0.1981
Rwork	0.196
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r9f
RMSD bond length	0.019
RMSD bond angle	2.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.020
High resolution limit [Å]	1.950	4.190	1.950
Rmerge	0.072	0.035	0.726
Number of reflections	32285
<I/σ(I)>	11
Completeness [%]	98.9	98.8	98.2
Redundancy	2.9	2.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	291	1.6 M ammonium sulfate, pH 8, potassium chloride, MES/NaOH, trace magnesium, HANGING DROP, temperature 291K